The Open Insulin Project

Lab intern role in engineering fermentation workflows and open-source protocols to produce low-cost insulin at Counter Culture Labs.

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Contributions

• Supported strain engineering by preparing media, performing mini-preps, digests, and selective plating under strict sterile conditions.
• Utilized ELISA assays, gene gun, electroporation, western blot, and bioreactors to optimize workflows.
• Familiarized with lab automation equipment and implemented automation in fermentation experiments.
• Streamlined DNA uptake methods, adopting simplified electroporation to improve yield.
• Collaborated with peers to refine open-source, low-cost insulin production protocols.